ABSTRACT
The antihyperglycaemic activity of IND 01 and its interaction with glyburide and pioglitazone on serum glucose, body weight and oral glucose tolerance test [OGTT] was determined in alloxan-induced diabetic mice. IND 01 [100 mg/kg], glyburide [10 mg/kg], pioglitzone [10 mg/kg] and their concomitant administration were administered orally in alloxan [80 mg/kg, i.v.] induced diabetic mice. The study design consisted of estimation of serum glucose after acute, subacute and glucose load administration. Administration of IND 01 [100 mg/kg] alone significantly [p<0.001] reduced serum glucose level at 6 h after administration. The antihyperglycaemic effect of glyburide and their concomitant administration of IND 01 with glyburide were similar, that is, onset was 2 h; peak effect was 6 h but the effect waned at 24 h. The onset of concomitant administration of IND 01 with pioglitazone was 4 h; peak effect was at 6 h but the effect waned at 24 h. In the subacute study, reduction in serum glucose was observed on 28[th] day after withdrawal for 7 days. The effects of concomitant administration were more pronounced than single drug treatment. In mice treated with either IND 01 [100 mg/kg], glyburide, pioglitazone alone or their combination, the body weight was not reduced in contrast to that in the control group. In the oral glucose tolerance test [OGTT], increased glucose utilization was observed in animals after concomitant administration of IND 01 [100 mg/kg] and glyburide [10 mg/kg] as well as IND 01 [100 mg/kg] and pioglitazone [10 mg/kg]. The concomitant administration of IND 01 with glyburide as well as pioglitzone produced synergistic antihyperglycaemic effect than either drug alone